AuTom: A novel automatic platform for electron tomography reconstruction.
R. Han, X. Wan, Z. Wang, Y. Hao, Y. Chen, X. Gao, X. Liu, F. Ren, F. Sun, F. Zhang
Journal of Structural Biology., 199(3), pp. 196-208, (2017)
Alignment, Electron tomography, Image processing workflow, Reconstruction
We have developed a software package towards automatic electron
tomography (ET): Automatic Tomography (AuTom). The presented package has
the following characteristics: accurate alignment modules for
marker-free datasets containing substantial biological structures; fully
automatic alignment modules for datasets with fiducial markers; wide
coverage of reconstruction methods including a new iterative method
based on the compressed-sensing theory that suppresses the "missing
wedge" effect; and multi-platform acceleration solutions that support
faster iterative algebraic reconstruction. AuTom aims to achieve fully
automatic alignment and reconstruction for electron tomography and has
already been successful for a variety of datasets. AuTom also offers
user-friendly interface and auxiliary designs for file management and
workflow management, in which fiducial marker-based datasets and
marker-free datasets are addressed with totally different subprocesses.
With all of these features, AuTom can serve as a convenient and
effective tool for processing in electron tomography.
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