S. Sagar, L. Esau, T. Hikmawan, A. Antunes, K. Holtermann, U. Stingl, V.B. Bajic, M. Kaur
BMC Complementary and Alternative Medicine, 13-29, (2013)
Deep sea brine pools,
High salinity and
temperature combined with presence of heavy metals and low oxygen
renders deep-sea anoxic brines of the Red Sea as one of the most extreme
environments on Earth. The ability to adapt and survive in these
extreme environments makes inhabiting bacteria interesting candidates
for the search of novel bioactive molecules.
Total 20 i.e. lipophilic
(chloroform) and hydrophilic (70% ethanol) extracts of marine bacteria
isolated from brine-seawater interface of the Red Sea were tested for
cytotoxic and apoptotic activity against three human cancer cell lines,
i.e. HeLa (cervical carcinoma), MCF-7 (Breast Adenocarcinoma) and DU145
Among these, twelve extracts
were found to be very active after 24 hours of treatment, which were
further evaluated for their cytotoxic and apoptotic effects at 48 hr.
The extracts from the isolates P1-37B and P3-37A (Halomonas) and P1-17B (Sulfitobacter) have been found to be the most potent against tested cancer cell lines.
Overall, bacterial isolates
from the Red Sea displayed promising results and can be explored further
to find novel drug-like molecules. The cell line specific activity of
the extracts may be attributed to the presence of different polarity
compounds or the cancer type i.e. biological differences in cell lines
and different mechanisms of action of programmed cell death prevalent in
different cancer cell lines.