M.E. D’Amato, V.B. Bajic, S. Davison
Sci Int Genet, 5(2):122-125, (2011)
The Y-chromosome STRs (short tandem repeat) markers are routinely
utilized in the resolution of forensic casework related to sexual
assault. For this, the forensic community has adopted a set of eleven
(core) Y-STR that is incorporated in all commercial diagnostic systems.
Our previous studies of Y-STR polymorphisms in the South African
population identified low levels of diversity and discrimination
capacity for many commercial marker sets, determining a limited
applicability of these systems to the local population groups. To
overcome this shortcoming, we designed a Y-STR 10-plex system that shows
higher discriminatory capacity (DC) than available commercial systems.
The markers were selected from a population group of 283 individuals
with African, European and Asian ancestry genotyped at 45 Y-STRs,
applying an optimization based selection procedure to achieve the
highest possible DC with the minimal number of markers. The 10-plex was
satisfactorily subjected to developmental validation tests following the
SWGDAM guidelines and shows potential for its application to
genealogical and evolutionary studies.