S. Meier, O. Ruzvidzo, M. Morse, L. Donaldson, L. Kwezi, C. Gehring
PLoS One, 5(1):e8904, (2010)
messengers have a key role in linking environmental stimuli to
physiological responses. One such messenger, guanosine 3′,5′-cyclic
monophosphate (cGMP), has long been known to be an essential signaling
molecule in many different physiological processes in higher plants,
including biotic stress responses. To date, however, the guanylyl
cyclase (GC) enzymes that catalyze the formation of cGMP from GTP have
largely remained elusive in higher plants.
have identified an Arabidopsis receptor type wall associated
kinase–like molecule (AtWAKL10) as a candidate GC and provide
experimental evidence to show that the intracellular domain of AtWAKL10431–700 can generate cGMP in vitro.
Further, we also demonstrate that the molecule has kinase activity
indicating that AtWAKL10 is a twin-domain catalytic protein. A
co-expression and stimulus-specific expression analysis revealed that AtWAKL10
is consistently co-expressed with well characterized pathogen defense
related genes and along with these genes is induced early and sharply in
response to a range of pathogens and their elicitors.
demonstrate that AtWAKL10 is a twin-domain, kinase-GC signaling
molecule that may function in biotic stress responses that are
critically dependent on the second messenger cGMP.